Chitin isolation results put into a beaker containing a solution of NaOH 50% w / v for 4 hours at a

Shrimp is a member of the phylum Arthropoda, sub-phylum Mandibulata and belonging to the class Crustacea. The entire body is made up of sections encased by outer skeleton combi steamer or exoskeleton of the substance horn or chitin and reinforced combi steamer by calcium carbonate limestone material. In shrimp waste, contained a biopolymer called chitin (poly-N Acetyl Glucosamine). Deacetylation of chitin will produce a more potent compounds, namely chitosan or poly [β - (1-4) -2-amino-2-deoxy-D-glucopyranose] glucosamine or D, with a certain degree of deacetylation. Chitosan lot of use, such as in the fields of pharmaceuticals, health, combi steamer agriculture and industry. Chitosan can also be used as coagulants and flocculants in water treatment (Mu'minah, 2008)
The process of isolation of chitin from shrimp shells can be broadly combi steamer divided into two, demineralization (mineral removal) and deproteinasi (removal of protein). If chitin is processed combi steamer further using concentrated alkaline solution it will produce a new product that chitosan. Chemically, chitin and chitosan can be considered as a derivative of cellulose with the hydroxyl group at C-2 atom cellulose DIGA ntikan by acetamide and free amine groups. If the hydroxy group at C-2 atom is replaced by cellulose acetamide group, the compound formed is chitin. But if the hydroxy group at C-2 cellulose atom replaced by a free amine group, combi steamer the compound formed is chitosan (Mia, 2007).
Chitosan which has the amine group is any element of N is highly reactive and is alkaline. The principle of chitosan coagulation is an amine salt ion exchangers which are formed due to reaction of amines with acid to exchange protons with electrons held metal contaminants owned by nitrogen (N). Chitosan is one example of the polyelectrolyte. Polyelectrolyte is part of a special polymer that can be ionized and have the ability to make the occurrence of flocculation in liquid medium combi steamer (Agus, et al, nd). Utilization of chitosan in water pengolaha combi steamer caused this compound acts as a chelating compound for pemisahkan heavy metals from solution combi steamer (Mu'minah, 2008).
At this stage the protein from shrimp waste is separated combi steamer by inserting 50 g of shrimp waste powder into 500 mL of NaOH solution 3.5% w / v and heated at 65 0 C for 2 hours, stirring constantly using a magnetic stirrer. Subsequently the mixture is cooled and filtered combi steamer with a filter cloth. The residue was filtered, washed deng's water until neutral and then rinsed with distilled water. Neutral residue which is a crude chitin dried in an oven at a temperature of 65 0 C for 24 hours and weighed. combi steamer
Shrimp waste protein that has been removed put in 1 M HCl solution at room temperature with a ratio of 1:15 w / v for 1 hour. Coarse chitin incorporated into the solution slowly. Mixing combi steamer process is done on top of a magnetic stirrer. The result of filtered reaction using a filter cloth. The filtered residue was washed with water until neutral and then rinsed with aqua dm. Neutral residue which is a coarse chitin, dried in an oven at a temperature combi steamer of 65 0 C for 24 hours and weighed.
Chitin isolation results put into a beaker containing a solution of NaOH 50% w / v for 4 hours at a temperature of 100 0 C at a ratio of 1:10 w / v. The mixture is stirred using a magnetic stirrer, temperature constant attempted. Results extract was filtered using a filter cloth chitin. Residue which is chitosan, washed with water until neutral and rinsed with distilled water. Chitosan was dried in an oven at a temperature of 65 0 C for 24 hours.
1. Agus Widodo, Mardianh, and Andy Prasetyo. Potential of Shrimp Chitosan as Coagulant Heavy Metals Liquid Waste Textile Industry. Department of Chemical Engineering, Institute of Technology November (ITS) Surabaya.
Shara Sherenia March 11, 2009 16:22
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