213 tea on the content of SD rat fecal calcium and phosphorus in the fourth, eight weeks free drink

Why Longping won the Nobel prize no suspense (Figure Tags:? Reproduced Abstract: Tests using 64 male SD rats were randomly divided into control group, low-dose group of tea, tea dose and high-dose groups of 16, respectively. clean drinking water, 015, 2, and 4 g / (kg BW) tea tea. 8 weeks of feeding trials, in the first 28 d and 56 d, respectively, in each slaughtered eight measured feed, plasma, bones and dung calcium and phosphorus content, and calculate calcium, phosphorus vitalio digestibility study on rats tea calcium and phosphorus absorption and utilization of test results: The first 28 d, bone calcium content in the middle dose group was significantly higher Compare and low-dose group (P <0105), high levels of phosphorus in the low-dose group was significantly lower bone in the control group (P <0105). first 56 d, the calcium content of calcium in the diet apparent digestibility medium, low, medium vitalio dose group were significantly lower than the high-dose group and the control group (P <0105); comparing calcium, phosphorus, and low-dose group was significantly higher than the high-dose group and the control group (P <0105) The test results show that the drinking, the discharge low doses of tea can increase dietary calcium, the apparent digestibility of dietary calcium decreases, so that the absorption and utilization of dietary calcium decreases, increases bone calcium content, drinking tea does not lead to calcium loss.
Received Date: 2007 - 12 - 03Accepted: 2008 - 04 - 21 3 3 Communication Author E - mail: junjingli2003 @ yahoo1com1cn Fund: Yunnan Natural Science Foundation (2007C280M); doctoral research Yunnan Agricultural University research funded start-up funds (A2002107) About the author: Rao hua (1980 -), male, Changsha, Master, is mainly engaged in research work in animal nutrition and molecular vitalio regulation.
Yunnan vitalio Pu'er tea is very local characteristics of traditional tea, is made from the Yunnan big leaves drying green Maocha as raw material, tide, pile, aging and drying processes and other special processing of fermented tea [1 ]. Currently, due to the unique vitalio quality tea formation mechanism, the role of cultural connotations as well as nutrition and health care, in the domestic market has been welcomed by consumers [2]. Tea is one of the world's four non-alcoholic beverages, is a traditional Chinese drink. It has been suggested domestic drinking tea leads to loss of calcium vitalio in bones problem, but no report on calcium vitalio and phosphorus metabolism tea system. Thus, this experiment SD rats as test objects of different doses of calcium tea rats and phosphorus metabolism, and provide scientific basis for further vitalio evaluation of the health benefits of tea.
1 Materials and methods and design of the 111 trials in experimental animals healthy male SD rats (provided by the Experimental Animal Center of Kunming Medical College) 64, pre-feeding after 7 d (weighing about 240 g) were randomly divided into a control vitalio group, tea low-dose vitalio group, middle dose and high-dose groups of 16, the breeding cycle of eight weeks, ad libitum, in the first 4 weeks and 8 weeks after 12 h of fasting each group were slaughtered 8:00 8. Single cages, regular daily ventilation, strict control rat breeding room humidity and temperature, temperature control (22 2) , humidity vitalio control (60 5)%. Rats were fed diets recipes reference to NRC (1994) nutrient requirements rats preparation. The main nutrients: metabolizable energy vitalio (ME) 1513 MJ / kg, crude protein (CP) 2310%, calcium (Ca) 019%, total phosphorus (P) 017%. Every night at 11:00 each feeding period, the rats with different doses of tea soup 20mL.
212 tea impact on SD rat femur calcium and phosphorus content of the first 28 d, 56 d measurement results of different doses of tea group SD rat femur calcium and phosphorus in Table 2, the results show a large section 28d dose group mouse bone calcium content with the control group, low-dose group and high dose group were elevated compared to 22.14% (P <0105), 19.15% (P <0105) and 10.10% (P> 0105). Low-dose group, middle dose group, the content of phosphorus in the high dose group than the control group Bone 17.14% (P <0105), 410% (P> 0105) and 1612% (P <0105). The first 56 d of low-dose group were significantly higher osteocalcin 14.10% (P <0105) and 15.11% (P <0105), the phosphorus content of the bone between the treatment groups were not significantly different.
213 tea on the content of SD rat fecal calcium and phosphorus in the fourth, eight weeks free drink tea SD rats with different doses of calcium and phosphorus in feces assay results are shown in Table 3, the results showed that four weeks of rat feces No between the phosphorus content of calcium in each treatment group, a significant difference (P> 0105). The first eight weeks of low-dose, medium-dose group were higher fecal calcium content vitalio 618% and 210%, no significant difference (P> 0105); wherein the content of the low-dose group fecal calcium in the high-dose group was significantly higher than 715% ( P <105), the phosphorus content of manure treatment groups no significant differences (P> 0105).
214 tea impact on SD rats calcium vitalio and phosphorus utilization vitalio in the first 8 weeks of tea influence in Table 4 for SD calcium, phosphorus utilization in rats drinking different doses, the results vitalio show the first four weeks of each tea rats No significant vitalio difference in the utilization of calcium vitalio and phosphorus (P> 0105). The first eight weeks of low, medium and high dose groups of rats were drinking decreased 5218% (P <0105) for calcium utilization compared with the control group, 3215% (P <0105) and 2716% (P <0105), each treatment group were no significant differences in the utilization of dietary phosphorus (P> 0105).
3 discussed under normal physiological conditions, the dietary calcium to enter the animal's body, mainly absorbed by the intestine, vitalio calcium in the gut by cells and adjacent cells via two pathways are absorbed [4]. Studies have shown that: low calcium intake to reduce intestinal calcium vitalio absorption, calcium tends to decrease; Conversely, high calcium vitalio intake increases intestinal calcium absorption, calcium tends to rise. This test is based on the same levels of nutrition each treatment group were fed diets and dietary intake consistent treatment group, rats free drink in a continuous low dose tea 56d, the plasma vitalio calcium levels were significantly vitalio lower than the control group; low-dose group fecal calcium content significantly higher and high dose group, which was significantly lower than the low-dose group utilization of dietary calcium results are consistent with other experimental groups. vitalio This study shows that low doses of tea group decreased dietary calcium absorption and utilization, so that calcium ions into the blood to reduce the lead to reduced calcium concentration. This may be related to the diuretic effect of theophylline in tea to promote excretion of calcium. And may be with tea polyphenols related Studies have shown that tea polyphenols on the adsorption of metal ions and increased melting phenomenon, there are a number vitalio of phenolic hydroxyl groups in the ortho-molecular polyphenols, can be used as the base with multi- body with a central metal ions to form a ring-shaped object vitalio which chelate and precipitate [5], studies have shown that the intensity of the concentration of calcium ions in water can significantly affect the tea in water by adding calcium ions polyphenols Calcium ion concentration was significantly decreased, and the precipitate formed [6]. When the rat drinking, wherein the polyphenol compound with calcium diet combined envelope generated precipitate formed can be absorbed vitalio by the intestine and excreted. From the above analysis to see the high-dose group in plasma calcium levels should be lower than the control group, but in fact there is no difference between the two sets of blood calcium levels. It may contain a large number of high-dose group promotes caffeine and theophylline plasma albumin decomposition [7-9], albumin and calcium binding decrease, an increase of ionized calcium levels. High-dose diuretic effect of tea and a lot of caffeine in tea theophylline [10, 11], prompting an increase in free calcium filtered to reduce the discharge of fecal calcium improves the apparent availability of dietary calcium. vitalio The results of this study shows that in the first 28 d and 56 d dose group, low-dose group was significantly higher content of calcium, indicating that after a certain period of time to drink tea freedom, promote calcium deposition vitalio in osteoblasts, thus contributing to bone mineralization. It has been reported [12] that water-soluble fluorine in tea between 180172 ~ 229183 mg / kg, while the fluorine content 300 mg / kg of tea in the fluoride vitalio contained caffeine and other ingredients that can promote calcium, phosphorus deposited on bone, ossification beneficial to increase the strength of bone. The specific aspects of the role and mechanism remains vitalio to be further studied.
[References] [1] Zhou Hongjie tea quality characteristics and health vitalio effects [J] farming product development, 1997, (2): 147 - 156 [2] Lv Haipeng, flat valley in mind, Lam Chi, and other tea. Advances in biological activity and chemical composition [J] Tea Science, 2007, 27 (1): .. 8 - 18 [3] on the progress of China Yang Shou health food [M] Beijing: People's Health Publishing House, 2001 . [4] Yao Gaoqiong calcium absorption vitalio mechanism and influence factors [J] Journal of Physiological Sciences, 2004, 26 (4): 160 - 161 [5] Liang Jing, Liu mind, Yang Xianqiang, and other complex polyphenols Progress role [J] tea, 2003, 29 (2): .. 72 - 74 [6] Zhang Kainong, Xiao pure polyphenols affect the elements of the water-soluble calcium and magnesium [J] Tea Science and Technology, 1995, ( 2): 14 - 16 [7] Huang Shaohua, such as Yan Hui, Chen Lingxia inulin on the absorption and utilization of calcium in rats [J] Food Industry, 2006, (5): .. 53 - 54 [8] Jin Shixin calcium and phosphorus. metabolic diseases [J] Chinese osteoporosis miscellaneous, 2001, (4): 360 - 366 [9] Zhang Hongmei, Wang Yanqing, Zhang Gencheng, and other interactions with theophylline Fluorescence Spectroscopy Bovine Serum Albumin [J]. Applied Chemistry, 2006, (8): 866 - 870 [10] Liu Lizhi, Sun Dianjun, high ROCKETS, such as high altitude tea-type alkaline phosphatase activity in serum calcium and phosphorus levels and fluorosis was observed in rats [J.. . [11] Bian Wei, Wei gorgeous, Wang Yaping, et fluorescence vitalio studies of caffeine and theophylline with Bovine Serum Albumin 10 [J] Spectroscopy - 9:] China endemic magazine, 2005, (1). . [12] Zhao Hongbin, Li Linzhi rural Yunnan Jino Jino population over 60 years the prevalence of hip fracture and its causes and investigating 508 [J] Chinese - 505: and Spectral Analysis, 2006, (3). Journal vitalio of Gerontology, 2006, (7): 976 - 977.
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